表1.ZFN、TALEN和CRISPR/Cas三种基因编辑工具特性
ZFN | TALEN | CRISPR/Cas | |
碱基识别 | 锌指结构 | 可变双残基重复 | sgRNA |
靶点 | 预测性差 | 预测性差 | 预测性高 |
引导分子 | 两个锌指结构对应 一个靶序列 | 两个TALEN结构对 应一个靶序列 | 一个sgRNA对应一 个靶序列 |
多靶点 | 困难 | 困难 | 较容易 |
可行性 | 一般大型公司有能力开展,成本高 | 耗时但成本可接受 | 易操作且成本低 |
如有问题欢迎联系作者交流:
(可上下滑动查看)
1.https://www.editforce.co.jp/en/news-en/release-en/2022/1027/
2.https://www.nature.com/articles/d42473-019-00327-w
3.https://www.editforce.co.jp/en/ppr/
4.Imai T, Yagi Y, Nakamura T. Recent Progress Toward RNA Manipulation with Engineered Pentatricopeptide Repeat Proteins in Applied RNA Bioscience (editors: Masuda S, Izawa S) pp. 151-160 (Springer, Singapore, 2018).
5.Kobayashi T, Yagi Y, Nakamura T. Development of genome dngineering tools from plant-specific PPR proteins using animal cultured cells. Methods Mol Biol 1469, 147-155 (2016).
6.https://www.editforce.co.jp/en/ppr/
7.Eid, A., Mahfouz, M. Genome editing: the road of CRISPR/Cas9 from bench to clinic. Exp Mol Med 48, e265 (2016). https://doi.org/10.1038/emm.2016.111
8.Hillary, V. E., & Ceasar, S. A. (2021). Genome engineering in insects for the control of vector borne diseases. Progress in Molecular Biology and Translational Science, 179, 197-223.
9.Cermak, T., Doyle, E. L., Christian, M., Wang, L., Zhang, Y., Schmidt, C., ... & Voytas, D. F. (2011). Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic acids research, 39(12), e82-e82.
10.Garneau, J. E., Dupuis, M. È., Villion, M., Romero, D. A., Barrangou, R., Boyaval, P., ... & Moineau, S. (2010). The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature, 468(7320), 67-71.
11.Mok, B. Y., de Moraes, M. H., Zeng, J., Bosch, D. E., Kotrys, A. V., Raguram, A., ... & Liu, D. R. (2020). A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature, 583(7817), 631-637.
12.Cho, S. I., Lee, S., Mok, Y. G., Lim, K., Lee, J., Lee, J. M., ... & Kim, J. S. (2022). Targeted A-to-G base editing in human mitochondrial DNA with programmable deaminases. Cell, 185(10), 1764-1776.
-End-更多精彩内容,点击下方视频号
为促进细胞与基因治疗领域的合作交流,更好的服务广大读者朋友,微信公众号“细胞与基因治疗领域”团队组建了专业的细胞与基因治疗行业交流群(涉及DNA药物、RNA药物和细胞治疗等方面),长按下方二维码,添加小编微信进群。由于申请人数较多,添加微信时请备注:院校/企事业单位名称—专业/职务—姓名。如果您是PI/ 教授/主管及以上职务,还请注明。
往期文章推荐: